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cdna encoding mbp  (New England Biolabs)


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    Structured Review

    New England Biolabs cdna encoding mbp
    Cdna Encoding Mbp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+encoding+mbp/pm24607360-58-1-13
    Average 86 stars, based on 1 article reviews
    cdna encoding mbp - by Bioz Stars, 2026-09
    86/100 stars

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    Plasmid Preparation:

    Article Title: PDZ affinity chromatography: a general method for affinity purification of proteins based on PDZ domains and their ligands.
    Article Snippet: The following cDNAs were codon-optimized for expression in Escherichia coli, synthesized, and inserted into the pJExpress414 expression vector (Cat. No. pJ414) by DNA 2.0 (Menlo Park, CA): Mus musculus GluN2B (residues 842–1482; Q01097) fused to maltose binding protein (MBP-GluN2B Tail); E. coli thioredoxin (THX) (P0AA25); Dasher Green Fluorescent Protein (DasherGFP; Custom Order, DNA2.0, Menlo Park, CA); Rattus norvegicus CRIPT (Q792Q4); residues 11–129 of M. musculus nNOS (Q9Z0J4), containing the PDZ domain (single PDZbh) or two fused copies of the PDZ domain (tandem PDZbh); and PDZ1 (residues 61–151), PDZ2 (residues 155–249), PDZ3 (residues 302–402) and PDZ1-2 (residues 61–249) of M. musculus PSD-95 (Q62108). .. A cDNA encoding MBP in pMAL-c4e plasmid (Cat. No. 8110) was purchased from New England Biolabs (Ipswitch, MA) and a cDNA encoding glutathione S-transferase (GST) in pGEX-6P-1 plasmid (Cat. No. 28- 9546-48) was purchased from General Electric Healthcare (Uppsala, Sweden). ..



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